• Surabhi Narvekar
• Abhishek Thilakan
• Mayuri Bhangale
• Ashwini Yeri
• Vaishnavi Pawar , Nikita Kasar, Pratiksha Shukla, Minal Trivedi

Xanthan are water soluble exopolysaccharides produced by Xanthomonas species. These Xanthmonas species were isolated from local infected samples i.e. cabbage leaf (Black rot) by Xanthomonas campestris and lemon (Citrus Canker) by Xanthomonas axonopodis and were confirmed on the basis of biochemical testing and Gram staining followed by its antibiotic sensitivity testing which gave zone of inhibition more than 20mm for some antibiotics like Chloramphenicol. These polysaccharides have much common applications and normally produced in submerged fermentation by using different carbon sources i.e. usage of different agro-industrial wastes. Xanthan is an ecofriendly and natural gum produced by using waste as fermentation substrate which can be alternative to other synthetic gums as well as traditional gums like agar, carrageenan, locust bean gum, gluten, guar gum which are obtained from aquatic algae (seaweeds) and plants hence disturbing natural environment. An attempt to synthesize Xanthan from agro-industrial wastes by submerged fermentation yielded a dry weight of 0.422gm/50mL of potato peels and 0.388gm/50mL of chickoo peel extract by Xanthomonas axonopodis whereas 0.558gm/50mL of potato peels and 0.398gm/50mL of chikoo peel extract by Xanthomonas campestris and the YDC standard gave 0.027gm/50mL and 0.043gm/50mL for Xanthomonas axonopodis and Xanthomonas campestris respectively. The work emphasizes the possibility of using agricultural waste as lower cost alternative substrates for Xanthan production which is widely used food additive and can be alternative for an agar

X. axonopodis, citrus canker, X. campestris, black rot, antibiotic sensitivity tests

"ISOLATION, IDENTIFICATION AND IN-VITRO ANTIBIOTIC SENSITIVITY PATTERN OF LOCAL ISOLATES OF XANTHOMONAS FOLLOWED BY PRODUCTION OF XANTHAN GUM USING AGRO-INDUSTRIAL WASTE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.181-188, May-2019, Available :http://www.jetir.org/papers/JETIRCB06028.pdf

"ISOLATION, IDENTIFICATION AND IN-VITRO ANTIBIOTIC SENSITIVITY PATTERN OF LOCAL ISOLATES OF XANTHOMONAS FOLLOWED BY PRODUCTION OF XANTHAN GUM USING AGRO-INDUSTRIAL WASTE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp181-188, May-2019, Available at : http://www.jetir.org/papers/JETIRCB06028.pdf