• Vishwa Uday Desai
• Dr. Reena G. Desai

Bioethanol is an alternative renewable fuel that can be produced from cellulosic biomass by hydrolysis and fermentation processes, for which Sugarcane bagasse (SCB) and Rice straw have been used in this study as they are cheap, readily available feedstock and possess high sugar content. For biological production of ethanol, agro-waste is enzymatically hydrolyzed to fermentable sugars. To increase this sugar content from substrate and to boost bioethanol production, for which along with enzymes, different pretreatment methods were studied. In this study three commercial enzymes, cellulase, xylanase and pectinase were used along with chemical pretreatment of NaOH and H2SO4 and then were optimized using response surface methodology for efficient hydrolysis. Further induction of bioethanol production was studied using fresh leaf, stem and fruit dried powder of Moringa oleifera. Heat, pressure and time were maintained constant during all pretreatments. Bioethanol production from treated biomass was performed by simultaneous scarification and fermentation (SSF) with different enzymes and their cocktail using yeast Saccharomyces cerevisiae. Optimal condition for SSF-27°C, 144h, Enzyme loading 1%, yeast inoculum 10 % (v/v) and substrate concentration 5% (w/v) were maintained. 5.2g/l bioethanol concentration was obtained using sugarcane bagasse (SCB) pretreated with 2% NaOH along with cocktail of all enzyme. In rice straw ethanol concentration of 3.36g/l was achieved with Xylanase enzyme and 2%NaoH pretreatment. Around 28.8% and 36.6% increase in production was induced using fresh leaf powder stem powder of Moringa oleifera in SCB and Rice straw respectively.

SCB, Rice straw, Bioethanol, Pretreatment, response surface modelling.

"Bioethanol production from agro-waste by pretreatment methods and optimization using Response surface methodology.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1160-1167, June-2020, Available :http://www.jetir.org/papers/JETIR2006165.pdf

"Bioethanol production from agro-waste by pretreatment methods and optimization using Response surface methodology.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1160-1167, June-2020, Available at : http://www.jetir.org/papers/JETIR2006165.pdf