• Shabir Ahmad Dar
• Manik Sharma
• Jagrati Tripathi
• Rajendra Chauhan
• Dixhya Rani

Diabetes mellitus a chronic metabolic disorder characterized by hyperglycaemia together with altered carbohydrate, lipid and protein metabolism due to inadequate amount of insulin secretion by pancreas. Various approaches are used to have a control/curb over this increased life threatened disease. Among these approaches, one of the most widely used one is, use of natural inhibitors which reduces gastrointestinal glucose production and absorption through the inhibition of carbohydrate digesting enzymes such as α- amylase and α-glucosidase. Through these strategic managements post prandial increase of blood glucose after a mixed carbohydrate diet can be reduced significantly. Keeping this therapeutic approach in mind we estimated the phytochemical contents of methanolic extract of M. vulgare L. leaf and its chloroform fraction and evaluate the vitro antidiabetic activity of chloroform fraction. The assay suggested that chloroform fraction containing highest content of terpenoids (636.81 ± 22.95 mg Le/g extract) exhibited dose dependent increase in inhibitory effect on α- amylase enzymes (IC50 487.33±2.65 g/ml) and α-glucosidase (IC50 392.95±7.38 g/ml). From the current study in which chloroform fraction (Chf fr) showing significant inhibitory effect on carbohydrate digestive enzymes, we depicted that the activity could be due to presence of terpenoids.

alpha-amylase, alpha-glucosidase, diterpenoids, total phenol content, total terpenoid content

"Quantitative estimation of phytochemicals and in vitro antidiabetic activity of chloroform fraction of Marrubium vulgare L. leaf.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.1168-1175, June-2019, Available :http://www.jetir.org/papers/JETIR1908D47.pdf

"Quantitative estimation of phytochemicals and in vitro antidiabetic activity of chloroform fraction of Marrubium vulgare L. leaf.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp1168-1175, June-2019, Available at : http://www.jetir.org/papers/JETIR1908D47.pdf