• Arti S. Bhanushali
• Sonali R. Mistry
• Dr. Krishpa B. Shah

Pollution is one of the major serious problem that is faced globally. Hydrocarbon contaminated sites possess a major threat to the environment. Release of hydrocarbon into the environment whether accidentally or due to human activities is the main cause of water and soil pollution. Biosurfactant are used in bioremediation of hydrocarbon contaminated sites and this could be a promising approach. With this view, isolation, screening and production of biosurfactant producing bacteria was investigated. In the present study primary screening was carried out using Mineral Salt agar plate containing 2% used engine oil as a substrate and a total of 12 morphologically distinct bacterial strains were isolated from different samples: Oil contaminated soil, Mangrove sediment and Marine water. Out of these 3 were Gram positive and 9 were Gram negative. S1, S2, S3, S4, S5, S6 isolates were obtained from oil contaminated soil, M1, M2 isolates from mangrove sediment and W1, W2, W3, W4 isolates were obtained from marine water. All of these isolates were then subjected for secondary screening: BATH assay, Drop collapse test, Oil displacement assay, Emulsification index and Phenol-sulfuric acid method. On the basis of all these test results best isolate from each type of sample was selected for the biosurfactant production. Then biosurfactant was extracted using acid-precipitation method and dry extracted biosurfactant was obtained.

Biosurfactants, BATH assay, Oil displacement, Phenol sulfuric acid, Extraction.

"ISOLATION, SCREENING AND PRODUCTION OF BIOSURFACTANT PRODUCING BACTERIA FROM OIL CONTAMINATED SOIL, MANGROOVE SEDIMENT AND MARINE WATER", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 7, page no.e796-e802, July-2021, Available :http://www.jetir.org/papers/JETIR2107604.pdf

"ISOLATION, SCREENING AND PRODUCTION OF BIOSURFACTANT PRODUCING BACTERIA FROM OIL CONTAMINATED SOIL, MANGROOVE SEDIMENT AND MARINE WATER", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 7, page no. ppe796-e802, July-2021, Available at : http://www.jetir.org/papers/JETIR2107604.pdf