UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 10 Issue 1
January-2023
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2301306


Registration ID:
507201

Page Number

d28-d41

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Title

Testicular and epididymal toxicity induced by high fat diet (HFD), alcohol, and their combination in male Wistar rats

Abstract

Obesity and alcohol abuse are life style diseases and both significantly involved in several reproductive pathologies contributing to infertility in men. The aim of this experiment was to study the independent and combinative effect of HFD and alcohol on bodyweight, relative reproductive tissues, sperm parameters, circulatory testosterone levels, testicular steroidogenic enzyme activities, antioxidant capacity in testes and epididymis in male wistar rats. A total of twenty-four male Wistar rats were randomly divided into 4 groups: (1) Control group (CON) fed with a commercial standard diet; (2) HFD, high fat diet group fed with a commercial beef chow containing 60% crude fat for 10 weeks; (3) Alcohol alone was given orally at 2 g/kg bw/day; (4) Co-administration of alcohol(2 g/kg) and HFD. Our resulted showed that rats in HFD group exhibited increased body weight gain compared to control. Further, markedly increase bodyweight gain in co-administration of HFD combined alcohol rats. On the other hand, there was statistically significantly decrease in bodyweight gain between alcohol alone treated group compared to control (p< 0.05). Moreover significant increase in relative weight of somatic tissues such as liver, kidney and brain was observed in HFD or co-administration of HFD combined alcohol compared to control rats. The relative weights of testes, epididymis, seminal vesicles, and prostate glands were significantly decreased in alcohol alone or combined HFD exposure rats compared to control rats (p< 0.05). Exposure to HFD or alcohol significantly decreased daily sperm production, motile-, viable- and HOS-tail swelled sperm, 3β- and 17β- hydroxysteroid dehydrogenase activity levels in testes of rats compared to control (p< 0.05). Similar trend was observed in co-administrated with HFD plus alcohol rats. Our results also showed that administration of rats with HFD or alcohol alone and combined alcohol and HFD caused a significant decrease of antioxidant enzyme (SOD, CAT, GSH-Px, and GR) activities and remarkable reduction in MDA content in testes, as well as in epididymis indicating that HFD alone or alcohol treated alone or alcohol plus HFD can increase oxidative stress in testes and epididymis. These findings suggest that HFD or alcohol has adverse effects on spermatogenesis and steroidogenesis. In addition, co-administrated HFD and alcohol rats showed higher reproductive toxicity than individual treatments. Finally, we conclude that consumption of alcohol combined with high calories diet leads to male infertility in rats.

Key Words

Alcohol, Obesity, Toxicity, High fat diet

Cite This Article

"Testicular and epididymal toxicity induced by high fat diet (HFD), alcohol, and their combination in male Wistar rats", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 1, page no.d28-d41, January-2023, Available :http://www.jetir.org/papers/JETIR2301306.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Testicular and epididymal toxicity induced by high fat diet (HFD), alcohol, and their combination in male Wistar rats", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 1, page no. ppd28-d41, January-2023, Available at : http://www.jetir.org/papers/JETIR2301306.pdf

Publication Details

Published Paper ID: JETIR2301306
Registration ID: 507201
Published In: Volume 10 | Issue 1 | Year January-2023
DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.32711
Page No: d28-d41
Country: Tirupati, Andhra Pradesh, India .
Area: Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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